anti-cd25 antibody clone pc-61  (BioExpress)

Journal: BMC Infectious Diseases

Article Title: Pre-treatment with IL2 gene therapy alleviates Staphylococcus aureus arthritis in mice

doi: 10.1186/s12879-020-4880-8

Figure Lengend Snippet: Summary of mouse experiments

Article Snippet: Three days before and 3 days after bacterial inoculation, C57BL/6 mice ( n = 10) or NMRI mice ( n = 10) were treated with an ip injection of 500 μg rat anti-mouse CD25 monoclonal antibody (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA) in a total volume of 100 μl of PBS.

Techniques: Infection, Adoptive Transfer Assay, Injection

Journal: BMC Infectious Diseases

Article Title: Pre-treatment with IL2 gene therapy alleviates Staphylococcus aureus arthritis in mice

doi: 10.1186/s12879-020-4880-8

Figure Lengend Snippet: Antibodies used for flow cytometry

Article Snippet: Three days before and 3 days after bacterial inoculation, C57BL/6 mice ( n = 10) or NMRI mice ( n = 10) were treated with an ip injection of 500 μg rat anti-mouse CD25 monoclonal antibody (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA) in a total volume of 100 μl of PBS.

Techniques:

Journal: BMC Infectious Diseases

Article Title: Pre-treatment with IL2 gene therapy alleviates Staphylococcus aureus arthritis in mice

doi: 10.1186/s12879-020-4880-8

Figure Lengend Snippet: Tregs in mice with S. aureus arthritis. a , Experimental set-up for the evaluation of Tregs proportions during the natural course of S. aureus arthritis. b , Tregs expressed as frequency of CD25 + FoxP3 + of the CD4 + lymphocytes in blood, spleen and lymph nodes in healthy uninfected mice and in mice with S. aureus arthritis. In panel B, data are shown as median, whiskers = min to max. Statistical calculations were made using the Mann-Whitney U-test and one-way ANOVA, ** P < 0.01, *** P < 0.001

Article Snippet: Three days before and 3 days after bacterial inoculation, C57BL/6 mice ( n = 10) or NMRI mice ( n = 10) were treated with an ip injection of 500 μg rat anti-mouse CD25 monoclonal antibody (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA) in a total volume of 100 μl of PBS.

Techniques: MANN-WHITNEY

Journal: BMC Infectious Diseases

Article Title: Pre-treatment with IL2 gene therapy alleviates Staphylococcus aureus arthritis in mice

doi: 10.1186/s12879-020-4880-8

Figure Lengend Snippet: Frequencies and T cell phenotypes at day 0, i.e. 19 days after inoculation of the rAAV-LUC/IL2 vectors. a , Gating strategies and frequency of CD25 + FoxP3 + out of the CD4 + lymphocytes in blood at day 0. b , Protein expression levels of CD25 and FoxP3 (measured as geometric mean fluorescence (gMFI)), in Tregs in rAAV-LUC and rAAV-IL2 treated animals. c , The frequency of CD4 + CD25 − Teff cells at day 0. d , The frequency of CD4 + CD25 + FoxP3 + activated Teff cells at day 0. Data are shown as median, whiskers = min to max. Statistical calculations were made using Mann-Whitney U-test, **** P < 0.0001

Article Snippet: Three days before and 3 days after bacterial inoculation, C57BL/6 mice ( n = 10) or NMRI mice ( n = 10) were treated with an ip injection of 500 μg rat anti-mouse CD25 monoclonal antibody (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA) in a total volume of 100 μl of PBS.

Techniques: Expressing, Fluorescence, MANN-WHITNEY

Journal: BMC Infectious Diseases

Article Title: Pre-treatment with IL2 gene therapy alleviates Staphylococcus aureus arthritis in mice

doi: 10.1186/s12879-020-4880-8

Figure Lengend Snippet: Effects of persistent IL2 production induced using an IL2-producing rAAV vector in S. aureus arthritis. a-b , Characteristics of CD4 + CD25 + FoxP3 + Tregs from peripheral blood and spleen a , Frequency measured during the course of S. aureus arthritis. b Protein expression levels of CD25 and FoxP3 (measured as geometric mean fluorescence (gMFI)), at day 10 after bacterial inoculation. c-g , Frequencies of other lymphocytes measured during the course of S. aureus arthritis in blood and spleen. c , CD4 + CD25 − Teffs d , CD4 + CD25 + FoxP3 − activated Teffs. e , NK1.1 + TCRβ − NK f , NK1.1 + TCRβ + NKT g , B cells h , CD8 + T cells. In panels a and c-h , bars show the mean ± standard error of the mean (SEM). In panel b data is shown as median, whiskers = min to max. Statistical calculations were made using Mann-Whitney U-test, * P < 0.05, **** P < 0.0001

Article Snippet: Three days before and 3 days after bacterial inoculation, C57BL/6 mice ( n = 10) or NMRI mice ( n = 10) were treated with an ip injection of 500 μg rat anti-mouse CD25 monoclonal antibody (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA) in a total volume of 100 μl of PBS.

Techniques: Plasmid Preparation, Expressing, Fluorescence, MANN-WHITNEY

Journal: BMC Infectious Diseases

Article Title: Pre-treatment with IL2 gene therapy alleviates Staphylococcus aureus arthritis in mice

doi: 10.1186/s12879-020-4880-8

Figure Lengend Snippet: Depletion of CD25 + Tregs. a , Experimental set-up for the depletion of Tregs using anti-CD25 antibodies and isotype control antibodies. b , Weight loss. c , Bacterial clearance in the kidneys. d , Clinical arthritis development. Depletion of CD25 + Tregs using anti-CD25 antibodies and isotype control antibodies in NMRI mice: e , Weight loss during the course of S. aureus arthritis. f , Bacterial clearance in the kidneys at day 10 after bacterial inoculation. g , Clinical arthritis development. In panels b , d , e and g , bars show the mean ± standard error of the mean (SEM). In panels c , and f , data are shown as median, whiskers = min to max. Statistical calculations were made using the Mann-Whitney U-test and one-way ANOVA, *P < 0.05

Article Snippet: Three days before and 3 days after bacterial inoculation, C57BL/6 mice ( n = 10) or NMRI mice ( n = 10) were treated with an ip injection of 500 μg rat anti-mouse CD25 monoclonal antibody (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA) in a total volume of 100 μl of PBS.

Techniques: Control, MANN-WHITNEY

Journal: BMC Infectious Diseases

Article Title: Pre-treatment with IL2 gene therapy alleviates Staphylococcus aureus arthritis in mice

doi: 10.1186/s12879-020-4880-8

Figure Lengend Snippet: Treatment with rhIL2 in S. aureus arthritis. a , Experimental set-up. Frequency of CD25 + FoxP3 + of CD4 + lymphocytes in b , spleen and c , blood at day 10 after bacterial inoculation. The frequencies of d , NK, e , NKT and f , B cells in blood 10 days after bacterial inoculation in mice after treatment with rhIL2. g , Weight loss. h , Bacterial clearance in the kidneys. i , Clinical severity of arthritis. In panels b , c and e , data are shown as median, whiskers = min to max. In panels d and f , bars show the mean ± standard error of the mean (SEM). Statistical calculations were made using the Mann-Whitney U-test, **P < 0.01, ***P < 0.01

Article Snippet: Three days before and 3 days after bacterial inoculation, C57BL/6 mice ( n = 10) or NMRI mice ( n = 10) were treated with an ip injection of 500 μg rat anti-mouse CD25 monoclonal antibody (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA) in a total volume of 100 μl of PBS.

Techniques: MANN-WHITNEY

Journal: International journal of cancer. Journal international du cancer

Article Title: CD4+ lymphocytes modulate prostate cancer progression in mice

doi: 10.1002/ijc.24452

Figure Lengend Snippet: Pre–Neoplastic Findings (In Dorsolateral Prostate) of Male Apc Min/+ (Min) Mice (Mean ± SE Per 10 20 × Images)

Article Snippet: Depletion of CD25 + cells A total of 16 (2 trials of 8 mice each) male Min mice aged 4–6 weeks were treated with anti-CD25 antibody (clone PC-61; Bio-Express, West Lebanon, NH) at 150 μg intraperitoneally per mouse 2 times weekly for 4–6 weeks.

Techniques: Control

Journal: International journal of cancer. Journal international du cancer

Article Title: CD4+ lymphocytes modulate prostate cancer progression in mice

doi: 10.1002/ijc.24452

Figure Lengend Snippet: Depletion of CD25+ cells increases the risk of prostate cancer in young mice, but complete lack of lymphocytes and increasing age are the major factors contributing to the progression of prostate neoplasia in ApcMin/+ mice. (a, b) HGPIN lesions in the anterior prostate of CD25-depleted Min mice at 3 months of age. Glands in a are distorted, surrounded by irregular fibromuscular sheaths and filled with atypical epithelial cells. The stroma adjacent to gland profile with PIN lesions in b contains neutrophils (open arrow-head) and mast cells (arrow-head). (c, d) Early invasive neoplastic lesions in the dorsolateral prostate of a RagMin mouse at 6 months of age. Highly irregular glands profiles with tufting epithelia and edematous stromal reaction (c). Higher magnification of the boxed area is shown in D. Early invasion of neoplastic epithelium is found in association with HGPIN. Note the hyperchromasia and the nuclear pleomorphism of the epithelial cells in PIN lesions (open arrow) and the large, euchromatinic nuclei bearing prominent nucleoli of invasive epithelial structures (arrow). (e, f) Prostate adenocarcinoma in the dorsolateral prostate of Min mice at 6 months of age. Moderately differentiated adenocarcinoma with desmoplastic reaction. Abnormal mitotic figure is indicated by arrow-head (e). Nuclear stabilization of β-catenin characterized advanced prostate adenocarcinoma lesions (f). (g, h) Prostate adenocarcinoma in the dorsolateral prostate of Min mice at 6 months of age. Well-differentiated adenocarcinoma with prominent desmoplasia (g). Increased numbers of mast cells (arrow-head) in stroma associate with abnormal glands (h). (a, b, c, d, e, g, h) Hematoxylin and Eosin. (f) β-catenin-specific immunohistochemistry; Hematoxylin counterstain, DAB chromogen. Bars a, c and g: 100 µm; b, d, e, f and h: 25 µm.

Article Snippet: Depletion of CD25 + cells A total of 16 (2 trials of 8 mice each) male Min mice aged 4–6 weeks were treated with anti-CD25 antibody (clone PC-61; Bio-Express, West Lebanon, NH) at 150 μg intraperitoneally per mouse 2 times weekly for 4–6 weeks.

Techniques: Immunohistochemistry

Journal: International journal of cancer. Journal international du cancer

Article Title: CD4+ lymphocytes modulate prostate cancer progression in mice

doi: 10.1002/ijc.24452

Figure Lengend Snippet: (a) Frequency of prostate cancer lesions in Min and Rag-deficient Min mice. Depletion of CD25+ cells (Min+anti-CD25) (N = 8 mice per trial) accelerated prostate carcinogenesis at age of 3 months, and the frequency of HGPIN and microinvasive adenocarcinoma was significantly higher than those of age-matched (N = 5 mice per trial) or sham-treated (N = 8) mice (p < 0.01 and <0.05, respectively). Compared to the age-matched Min mice (N = 5), RagMin mice (N = 8) had significantly higher frequency of prostate neoplasia at 6 months old (p < 0.05). Assays used tissues from 5 to 8 mice per treatment group (as shown) with review of microscopic fields as described in Material and methods. (b) Histology of dorsolateral prostate illustrating selected key intermediate steps in progression of carcinogenesis in ApcMin/+ mice. (a) Normal-appearing prostate gland. (b) Low-grade prostatic intraepithelial neoplasia (PIN) with epithelial tufting and nuclear stratification. Cellular atypia is evidenced by the presence of nuclear enlargement, increased but not severe nuclear pleomorphism, hyperchromasia and occasional prominent nucleoli. (c) High-grade PIN. Note the irregular contour of the prostate gland. Highly atypical cells with severe nuclear pleomorphism and hyperchromasia fill the lumen. (d) Microinvasive carcinoma. Epithelial cells with notably large, euchromatinic nuclei bearing prominently enlarged nucleoli with penetration through the basement membrane into surrounding stroma. (e) Invasive adenocarcinoma. Moderately differentiated small, irregular malignant glands are bounded by desmoplastic stroma. Hematoxylin and Eosin. Bars: 25 µm. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]

Article Snippet: Depletion of CD25 + cells A total of 16 (2 trials of 8 mice each) male Min mice aged 4–6 weeks were treated with anti-CD25 antibody (clone PC-61; Bio-Express, West Lebanon, NH) at 150 μg intraperitoneally per mouse 2 times weekly for 4–6 weeks.

Techniques: Membrane

Journal: International journal of cancer. Journal international du cancer

Article Title: CD4+ lymphocytes modulate prostate cancer progression in mice

doi: 10.1002/ijc.24452

Figure Lengend Snippet: mRNA expression levels of IL-6 and Cox-2 in the murine prostate and bowel. There were significant increases in expression of IL-6 in (a) prostate and (b) ileum of Min mice treated with anti-CD25 antibody, and also in Rag-deficient Min mice, when compared to sham-treated Min counterparts. Supplementation with TREG cells from wt donor mice decreased expression of IL-6 in prostate tissue, whereas depletion of CD25+ cells increased IL-6 gene expression in prostate tissue. Assay used tissues from 5 to 8 mice per treatment group. For comparison of mRNA levels, the target mRNA was normalized to that of the housekeeping gene GAPDH. Numbers on the y-axis represent mean fold change of target mRNA levels in reference to the control levels (B6 wt, defined as 0, standard deviation represented by solid bars). mos, age in months upon necropsy.

Article Snippet: Depletion of CD25 + cells A total of 16 (2 trials of 8 mice each) male Min mice aged 4–6 weeks were treated with anti-CD25 antibody (clone PC-61; Bio-Express, West Lebanon, NH) at 150 μg intraperitoneally per mouse 2 times weekly for 4–6 weeks.

Techniques: Expressing, Gene Expression, Comparison, Control, Standard Deviation

Journal: International journal of cancer. Journal international du cancer

Article Title: CD4+ lymphocytes modulate prostate cancer progression in mice

doi: 10.1002/ijc.24452

Figure Lengend Snippet: Immunohistochemical staining illustrates critical steps of carcinogenesis in the Min mouse model of prostate carcinoma. The aberrant immunostaining pattern observed in PIN lesions of Min mice that were depleted of CD25+ cells (right row) can be appreciated by comparison with normal prostate epithelia of wildtype mice (left row). (a–d) Dorsolateral prostate. (e–h) Anterior prostate. (a, b) The normal cytoplasmic expression of Apc (A) is absent in Min mouse prostate shown in b. (c, d) SMA immunohistochemistry highlights the fibromuscular sheath of prostate glands. Compare the smooth and continuous contour of normal prostate gland in c with the irregular and discontinuous (arrow-heads) sheath of prostate gland in d. (e, f) Normal lateral epithelial cell membrane staining pattern of E-cadherin in normal prostate (e) is by large lost in HGPIN shown in f. (g, h) Normal immunolabeling of β-catenin is largely restricted to adherence junctions in the prostate of wildtype mice (g). HGPIN lesions (h) instead of normal staining pattern show intense cytoplasmic staining, which indicates accumulation of β-catenin in the cytoplasm of abnormal prostate epithelial cells. Hematoxylin counterstain, DAB chromogen. Bars: 25 µm.

Article Snippet: Depletion of CD25 + cells A total of 16 (2 trials of 8 mice each) male Min mice aged 4–6 weeks were treated with anti-CD25 antibody (clone PC-61; Bio-Express, West Lebanon, NH) at 150 μg intraperitoneally per mouse 2 times weekly for 4–6 weeks.

Techniques: Immunohistochemical staining, Staining, Immunostaining, Comparison, Expressing, Immunohistochemistry, Membrane, Immunolabeling

Journal: International journal of cancer. Journal international du cancer

Article Title: CD4+ lymphocytes modulate prostate cancer progression in mice

doi: 10.1002/ijc.24452

Figure Lengend Snippet: Depletion of lymphocytes correlates with an invasive neoplastic phenotype in the small intestine of Apcmin/+mice. (a) Ampullary cancer arising from pancreatic duct epithelium in 25% (2/8) of 3-month-old ApcMin/+ mice after undergoing depletion of CD25+ cells (N = 8 mice per trial). High magnification (inset in a) reveals features of the abnormal pancreatic duct epithelium including pseudostratification and nuclear pleomorphism with associated inflammation. (b) Ileum of Rag2−/− ApcMin/+ mouse at 6 months of age revealing submucosal and incipient tunica muscularis invasion of neoplastic glands in an adenomatous polyp. Large numbers of mast cells infiltrate the submucosa and muscle layers at the base of the polyp. The higher magnification (inset) reveals the close topographic association of mast cells with the invasive front of the tumor. (a) Hematoxylin and Eosin. (b) Toluidine Blue. Bars a and b: 250 µm; insets: 25 µm. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]

Article Snippet: Depletion of CD25 + cells A total of 16 (2 trials of 8 mice each) male Min mice aged 4–6 weeks were treated with anti-CD25 antibody (clone PC-61; Bio-Express, West Lebanon, NH) at 150 μg intraperitoneally per mouse 2 times weekly for 4–6 weeks.

Techniques:

Journal: Clinical and Experimental Immunology

Article Title: Depletion of regulatory T cells in a hapten-induced inflammation model results in prolonged and increased inflammation driven by T cells

doi: 10.1111/cei.12466

Figure Lengend Snippet: Overview of the antibodies and compounds specifying clone, dose, depletion check and days of dosing in relation to sensitization on day −5 and challenge on day 0

Article Snippet: Antibody treatment On days −16 and −13 mice were treated with 200 μg rat anti-mouse CD25 monoclonal antibody (mAb) intraperitoneally (i.p.) (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA).

Techniques: Recombinant

Journal: Clinical and Experimental Immunology

Article Title: Depletion of regulatory T cells in a hapten-induced inflammation model results in prolonged and increased inflammation driven by T cells

doi: 10.1111/cei.12466

Figure Lengend Snippet: Depletion of regulatory T cells (Tregs) with a rat anti-mouse CD25 monoclonal antibody. (a) Mice were treated with rat anti-mouse CD25 monoclonal antibody (mAb) on days −16 and −13 prior to challenge. Depletion was confirmed using intracellular forkhead box protein 3 (FoxP3)-staining followed by flow cytometric analysis on days −12, −5 and 10. Mice were subsequently sensitized on day −5, challenged on day 0 and the ear-swelling response was measured 10 days post-challenge. (b) %CD25+FoxP3+ cells of CD4+ cells in blood checked by flow cytometry at days −12, −5 and 10 in relation to challenge in groups treated with 100, 200 and 400 μg anti-CD25 mAb/mouse, respectively, as well as in a untreated control group. (c) Ear-swelling response in groups treated with 0 (untreated control), 100, 200 and 400 μg anti-CD25 mAb/mouse in 10 days post-challenge. (d,e) %CD4+ cells of CD45+ cells in the blood at days −12 (d) and −5 (e) in groups treated with 100, 200 and 400 μg anti-CD25 mAb/mouse, respectively, as well as in an untreated control group. Data are depicted as mean ± standard error of the mean, n = 5/group.

Article Snippet: Antibody treatment On days −16 and −13 mice were treated with 200 μg rat anti-mouse CD25 monoclonal antibody (mAb) intraperitoneally (i.p.) (clone: PC.61·5, rIgG1; BioXcell, West Lebanon, NH, USA).

Techniques: Staining, Flow Cytometry, Control